Composite

Part:BBa_K3570003:Design

Designed by: Anton Mykhailiuk   Group: iGEM20_Toulouse_INSA-UPS   (2020-10-23)


Lemon taste induction system in S. cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3900
    Illegal XbaI site found at 3238
    Illegal XbaI site found at 3629
    Illegal PstI site found at 137
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3900
    Illegal PstI site found at 137
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3900
    Illegal BglII site found at 524
    Illegal BamHI site found at 3246
    Illegal XhoI site found at 5695
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3900
    Illegal XbaI site found at 3238
    Illegal XbaI site found at 3629
    Illegal PstI site found at 137
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3900
    Illegal XbaI site found at 3238
    Illegal XbaI site found at 3629
    Illegal PstI site found at 137
    Illegal NgoMIV site found at 3519
    Illegal AgeI site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

YPRctau3 up/down - S. cerevisiae genome; Limonene synthase- Citrus limon genome; Gal 1/10 promoter - S. cerevisiae genome; PGK terminator - S. cerevisiae genome; MET17 selective locus - S. cerevisiae genome;

References